复方人参皂苷纳米乳的制备及其急性毒性研究

doi:10.11843/j.issn.0366-6964.2014.04.023

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (4): 666-671.doi: 10.11843/j.issn.0366-6964.2014.04.023

复方人参皂苷纳米乳的制备及其急性毒性研究

曹发昊^1,2,欧阳五庆^2*,王艳萍¹

（1.运城学院生命科学系，运城 044000；2.西北农林科技大学动物医学院，杨凌 712100）

收稿日期:2013-09-30 出版日期:2014-04-23 发布日期:2014-04-23
通讯作者: 欧阳五庆（1960-），教授，主要从事纳米生物技术和新兽药开发研究
作者简介:曹发昊（1981-），男，河南辉县人，博士，主要从事纳米药物和天然活性成分研究，E-mail：fahaocao@163.com
基金资助:
陕西省重大科技创新专项基金（K332020916）；运城学院院级科研项目（CY-2012005）

Study on the Preparation and Acute Toxicity of Nanoemulsion Containing Ginsenoside and Levamisole Hydrochloride

CAO Fa-hao^1,2，OU YANG Wu-qing^2*，WANG Yan-ping¹

(1.Department of Life Sciences，Yuncheng University，Yuncheng 044000， China;2.College of Veterinary Medicine，Northwest A&F University，Yangling 712100， China)

Received:2013-09-30 Online:2014-04-23 Published:2014-04-23

摘要/Abstract

摘要：

利用纳米化技术，将人参皂苷和盐酸左旋咪唑组成复方人参皂苷纳米乳，对其组成和急性毒性进行研究。利用伪三元相图筛选出用于制备复方人参皂苷纳米乳的空白纳米乳；研究不同含药量纳米乳对免疫抑制小鼠脾T淋巴细胞增殖的影响，根据免疫增强作用较强的含药纳米乳，确定复方人参皂苷纳米乳的组成；分别用透射电镜、激光粒度仪和小鼠灌胃试验对其形态、粒径和急性毒性进行研究。结果显示，确定的复方人参皂苷纳米乳组成：Solutol^®HS-15、甘油、PEG400、IPM、蒸馏水，其质量比为25.33∶10.14∶2.53∶6∶56，GS和LH含量均为30 mg·mL^－1；它是黄色透明O/W纳米乳，液滴呈球形，平均粒径为23.08 nm，粒度分散指数为0.237，小鼠灌胃LD₅₀为402.85 mg·kg^－1。本试验成功制备了复方人参皂苷纳米乳，为进一步开发纳米化免疫增强剂提供了依据。

Abstract:

By using nanotechnology，ginsenoside(GS) and levamisole hydrochloride(LH) were combined with the carrier nanoemulsion to prepare compound GS and LH nanoemulsion(GS-LH-NE).Then its composition and acute toxicity were evaluated.The optimum blank nanoemulsion，which was used to prepare GS-LH-NE，was screened out through pseudo-ternary phase diagram.The enhancement effect of nanoemulsion containing different content of drug on the immunologic function of immunosuppressive mice were studied through splenic lymphocytes proliferation test，and the nanoemulsion with a better immunologic enhancement effect was screened out for ascertaining the composition of GS-LH-NE.The morphology of GS-LH-NE was observed using transmission electron microscope; the particle size was measured using laser particle size analyzer; the gavage acute toxicity of GS-LH-NE was measured using mice.The ascertained composition of GS-LH-NE was Solutol^®HS-15/glycerol/PEG400/IPM/distilled water(with the mass ratio of 25.33:10.14:2.53:6:56)，in which the content of GS and LH were both 30 mg·mL^－1and 30 mg·mL^－1，respectively.GS-LH-NE was a yellow transparent oil-in-water nanoemulsion，which had spherical droplets with the particle size of 23.08 nm and PDI of 0.237，and its gavage LD₅₀ was 402.85 mg·mL^－1.GS-LH-NE is successfully prepared and provides the possibility to develop immunologic enhancer through nanotechnology.

中图分类号:

S859.53

曹发昊,欧阳五庆,王艳萍. 复方人参皂苷纳米乳的制备及其急性毒性研究[J]. 畜牧兽医学报, 2014, 45(4): 666-671.

CAO Fa-hao，OU YANG Wu-qing，WANG Yan-ping. Study on the Preparation and Acute Toxicity of Nanoemulsion Containing Ginsenoside and Levamisole Hydrochloride[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 666-671.

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复方人参皂苷纳米乳的制备及其急性毒性研究

Study on the Preparation and Acute Toxicity of Nanoemulsion Containing Ginsenoside and Levamisole Hydrochloride

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